General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress OPRK1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0088E2CP2L)
The cAMP Hunter OPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of OPRK1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRK1, a naturally Gi/Go coupled GPCR. Upon activation of OPRK1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress MC4R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0050E2CP2L)
The cAMP Hunter MC4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC4R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC4R, a naturally Gs coupled GPCR. Upon activation of MC4R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CSF3R-JAK1 Functional Assay, 2-Plate Kit. (93-0809E3CP16M)
The PathHunter CSF3R-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses CSF3R activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged CSF3R cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Echelon Biosciences Research Labs AUTOTAXIN ACTIVITY ASSAY KIT
Echelon Biosciences - Autotaxin Activity Assay This item is non returnable
Eurofins Discoverx cAMP Hunter™ Human DRD5 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human DRD5 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD5 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Rat TAAR1 Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Rat TAAR1 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure rat TAAR1 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human Glucagon (GCG Receptor) β-Arrestin-1 Cell-Based Assay Kit (CHO-K1)
The PathHunter Human Glucagon (GCGR) β-Arrestin-1 Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GCGR (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human MDC (CCR4) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human MDC (CCR4) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human OPRM1 (Opioid mu) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human OPRM1 (Opioid mu) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Medchemexpress LLC cGAS Human His 100ug
cGAS is a cytosolic DNA sensor cGAS binds double-stranded DNA (dsDNA) and catalyzes the generation of cyclic guanosine-adenylate (cGAMP) which in turn activates the STING protein cGAS induces the production of type I interferon (IFN-I) and pro-inflammatory cytokines cGAS Protein Human (His) is a recombinant cGAS protein expressed in E coli with a C-6 His tag
Abcam DNMT Activity Assay Kit (Fluorometric)
DNMT Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology LTB4R Rabbit pAb
Predicted to enable G protein-coupled peptide receptor activity and leukotriene B4 receptor activity. Predicted to be involved in inflammatory response and neuropeptide signaling pathway. Predicted to act upstream of or within signal transduction. Predicted to be located in plasma membrane. Predicted to be integral component of plasma membrane.
Eurofins Discoverx PathHunter eXpress PTAFR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0236E2CP0L)
The PathHunter PTAFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTAFR and EA-tagged β-Arrestin-2. Activation of PTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC QuickZyme Human Granzyme B Activity Assay Reagent Set 2 X 96-Assays
QuickZyme Human Granzyme B Activity Assay Reagent Set for 2 x 96 assays. 96-well plate format; contains antibody, standard, detection enzyme, substrate. Quantitative measurement of ACTIVE human Granzyme B. Sample type: conditioned cell culture media, cell extracts, body fluids, tissue extracts, purified enzyme preparations. Also recognizes mouse Granzyme B. Can detect both high and low ranges. Dynamic range 0.05-10 ng/ml. Sensitivity 0.01 ng/ml (24 hr incub.). Sample vol. 10-100 ul. Granzymes are exogenous serine proteinases released from cytoplasmic granules of CTLs and NK cells. Granzyme B can act extracellularly (independent of perforin) and influences ECM degradation/remodeling by cleavage of ECM proteins, including laminin, decorin, fibronectin and vitronectin. Thus, Granzyme B may play an important role in wound healing and inflammatory diseases involving ECM degradation. Synonyms: C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Fragmentin-2, Granzyme-2, lymphocyte protein, HLP, SECT.